DLD-1（人結直腸腺癌細胞）
CBP60037
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| I. General information | |
| Synonyms: | DLD-1 |
| Background: | DLD-1 is one of two colorectal adenocarcinoma cell lines which were isolated by D.L. Dexter and associates during a period from 1977-1979 |
| Species: | Homo sapiens, human |
| Tissue: | colon |
| Disease: | Dukes' type C, colorectal adenocarcinoma |
| Gender: | male， adult |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,11 D16S539: 12,13 D5S818: 13 D7S820: 10,12 THO1: 7,9.3 TPOX: 8,11 vWA: 18,19 Cobioer’s Cell Line Authentication Service |
| Isoenzymes： | ES-D, 1-2 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1 |
| Culture Medium: |
RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | Blood type O. The cells are weakly positive for keratins and vimentin. The cells are positive for keratin by immunoperoxidase staining. DLD-1 cells are positive for p53 antigen expression (the p53 antigen produced has a C -> T mutation resulting in Ser -> Phe at position 241). |
| Oncogene: | myc +; myb + ; ras +; fos +; sis +; p53 +; abl -; ros -; src - |
| Genes Expressed: | carcinoembryonic antigen (CEA) 0.5 ng/106 cells/10 days; colon antigen 3. |
| Cellular Products： | carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; colon antigen 3; keratin |
| Tumor Formation: | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments: | This cell line is one of four colorectal adenocarcinoma cell lines that have been identified as being derived from a single individual. DNA profiling studies [1,2] have shown that DLD-1 (ATCC CCL-221), HCT-15 (ATCC CCL-225), HCT-8 (ATCC CCL-244) and HRT-18G (ATCC CRL-11663) share a single profile. DNA fingerprinting and cytogenetic analyses performed at ATCC and elsewhere show the line is similar to HCT-15 (CCL-225) and suggest the two are of different clonal origin from the same individual. Their genetic origin has been confirmed by DNA fingerprinting; however, cytogenetic analysis has shown that they lack concurrent marker chromosomes or concurrent numerical changes. A culture of unknown passage submitted to the ATCC in 1979 was found to be contaminated with Mycoplasma hyorhinis. The cells were subsequently cured using a combination of antibiotics over a 12-week cultivation period. Following treatment, the cells were assayed by the Hoechst stain weekly and by the standard culture test periodically. During 11 consecutive months of cultivation in the absence of antibiotics, all of these tests were negative |
