C2BBe1（人結腸癌細胞）
CBP60007
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| I. General information | |
| Cell Name: | C2BBe1 |
| Background: | The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line by limiting dilution; the clone was selected on the basis of morphological homogeneity and exclusive apical villin localization; C2BBe1 cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon; isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II ; the cells express substantial levels of BB myosin I similar to that of the human enterocyte; although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins. |
| Species: | Homo sapiens, human |
| Tissue: | Colon |
| Disease: | Colorectal adenocarcinoma |
| Gender: | Male, 72 years, Caucasian |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| DNA Profile: |
Amelogenin: X |
| Culture Medium: |
MEM+20%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90% Complete Medium+10%DMSO |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
