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CF-1 MEF(小鼠胚胎成纖維細胞)

CBP61128

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I. General information 
Synonyms: CF-1 MEF
Background: The cell line was established by ATCC in 2003 from embryonic day 14 (E14) CF-1 mouse embryos.
Species: Mus musculus, mouse
Tissue: Embryo
Disease: N/A 
Gender: 14 days gestation embryo, male and female mixed
Morphology: Fibroblast
Growth Mode: adherent
Doubling Time: N/A
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

DMEM+10%FBS+ 1%Non Essential Amino Acids (NEAA)

CF-1 MEF完全培養基,# CBP61128M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: N/A
Receptor Expression: N/A
Oncogene: N/A
Genes Expressed: N/A
Cellular Products: N/A
Tumor Formation: N/A
Comments: The growth of these cells should be arrested before being used as a feeder layer. ATCC has successfully irradiated (SCRC-1040.1™) and treated the cells with Mitomycin C (SCRC-1040.2a™) for use as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 6 (P6).
For more information, please contact Cobioer (4008-750-250).

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