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BALB/3T3 clone A31(小鼠胚胎成纖維細胞)

CBP60866

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I. General information 
Synonyms: BALB/3T3 clone A31
Background: The BALB/3T3 clone A31 is one of several cell lines (see ATCC CCL-164, BALB/3T12) developed by S.A. Aaronson and G.T. Todaro in 1968 from disaggregated 14- to 17-day-old BALB/c mouse embryos.
Species: Mus musculus, mouse
Tissue: embryo
Gender: embryo; 14 to 17 days gestation
Morphology: fibroblast
Growth Mode: adherent
Culture Medium:

DMEM+10%FBS

BALB/3T3 clone A31完全培養基,# CBP60866M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Tumor Formation: No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.                      
No, in immunosuppressed mice
Yes, in semisolid medium
Comments: Virus Susceptibility Herpes simplex virus
Vesicular stomatitis virus                                                                                            Virus Resistance {265A7263-515F-42F9-89FD-9B140DF21BEC}   
The cells are extremely sensitive to contact inhibition of cell division, grow at a high dilution, exhibit a low saturation density and are highly susceptible to transformation in tissue culture by the oncogenic DNA virus, SV40, and murine sarcoma virus
Tested and found negative for ectromelia virus (mousepox).
For more information, please contact Cobioer (4008-750-250).

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