U-937（人組織細胞淋巴瘤細胞）
CBP60277
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| I. General information | |
| Synonyms: | U-937 |
| Background: | The U-937 cell line was derived by Sundstrom and Nilsson in 1974 from malignant cells obtained from the pleural effusion of a patient with histiocytic lymphoma. |
| Species: | Homo sapiens, human |
| Tissue: | Pleura/pleural effusion, lymphocyte, Myeloid |
| Disease: | histiocytic lymphoma |
| Gender: | male, 37 years, Caucasian |
| Morphology: | monocyte |
| Growth Mode: | suspension |
| DNA Profile: | Amelogenin: X CSF1PO: 12 D13S317: 10,12 D16S539: 12 D5S818: 12 D7S820: 9,11 THO1: 6, 9.3 TPOX: 8,11 vWA: 14, 15 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS U-937完全培養基，# CBP60277M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Receptor Expression: | complement (C3) |
| Genes Expressed: | lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA) |
| Cellular Products: | lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA) |
| Comments: | Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocks of U-937 were contaminated with the human myeloid leukemia cell line, K-562. In the earliest stocks available, the level of contamination was 0.6%. Distribution was discontinued in March 1994, except if required for patent purposes. Anyone who wishes to receive a sample of this original material should contact the Head of the ATCC Patent Depository. A stock of CRL-1593 found to be free of K-562 was propagated continuously for 8 weeks and tested weekly by PCR. Distribution and seed stocks give DNA profiles characteristic of U-937 only. Such preparations are now offered as authentic U-937 (ATCC CRL-1593.2) and are believed to be free of second subpopulations. For more information, please contact Cobioer (4008-750-250). |
