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KARPAS 299(人間變性大細胞淋巴瘤細胞株)

CBP60271

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I. General information 
Synonyms: KARPAS 299
Background: A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin's lymphoma in 1986; now classed as CD30+ anaplastic large cell lymphoma (ALCL); cells carry the NPM-ALK fusion gene.
Species: human (Homo sapiens)
Tissue: Lymphoma
Disease: Human Non-Hodgkin's Ki-positive Large Cell Lymphoma
Gender: 25-year-old, male
Morphology: Lymphoblastoid
Growth Mode: Suspension
Doubling Time: N/A
DNA Profile: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 7,8.3
TPOX: 8
vWA: 17,19
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+10%FBS

KARPAS 299完全培養基,# CBP60271M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Hypodiploid
Subculture Routine: Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.
Comments: For more information, please contact Cobioer (4008-750-250).

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