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NCI-H460(人大細胞肺癌)

CBP60138

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I. General information 
Synonyms: NCI-H460
Background: The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung.
Species: Homo sapiens, human
Tissue: lung: pleural effusion
Disease: carcinoma; large cell lung cancer
Gender: male
Morphology: epithelial
Growth Mode: adherent
Doubling Time: 23 hrs in medium with serum; 42 to 60 hrs in serum
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 13
D16S539: 9
D5S818: 9,10
D7S820: 9,12
THO1: 9.3
TPOX: 8
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+10%FBS

NCI-H460完全培養基,# CBP60138M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Tumor Formation: Yes, in nude mice
Comments: The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities
The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.
For more information, please contact Cobioer (4008-750-250).

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