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MFE-296(人子宮內膜腺癌細胞)

CBP60840

產(chǎn)品描述
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I. General information 
Synonyms: MFE-296
Background: established from the primary tumor of a 68-year-old Caucasian woman with moderately differentiated endometrial adenocarcinoma (grade G2) in 1991; cells were described in the literature to express androgen receptors (androgen was reported to inhibit cell proliferation), but no estrogen receptors; cells were described in the literature to be tumorigenic in nude mice
Species: human (Homo sapiens)
Tissue: endometrium  
Disease: endometrium adenocarcinoma
Gender: N/A 
Morphology: epithelial-like polymorphic cells growing adherently in monolayers
Growth Mode: N/A 
Doubling Time: ca. 50-60 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)

MFE-296完全培養基,# CBP60840M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays 
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of LDH, NP
Cytogenetics: human diploid karyotype with 20% polyploidy - 46<2n>XX, no consistent abnormality detected - matches published karyotype
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/I I-, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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