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SK-N-BE(2)(人神經(jīng)母細胞瘤細胞)

CBP60743

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I. General information 
Synonyms: SK-N-BE(2)
Background: The SK-N-BE(2) neuroblastoma cell line was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.
Species: Homo sapiens, human
Tissue: brain; derived from metastatic site: bone marrow
Disease: neuroblastoma
Gender: male,  2 years
Morphology: neuroblast
Growth Mode: mixed, adherent and suspension
Doubling Time: 30 hrs
DNA Profile: Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 6,7
TPOX: 8,11
vWA: 18
Cobioer’s Cell Line Authentication Service
Culture Medium:

MEM:F12(1:1)+10%FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)

SK-N-BE(2)完全培養基,# CBP60743M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Tumor Formation: Yes, an inoculum of 107 cells produced tumors in cortisonized hamster cheek pouches with 18% frequency
Comments: The cells exhibit moderate levels of dopamine beta hydroxylase activity.
SK-N-BE(2) cells have a reported saturation density greater than 1 X 106 cells/cm2.
The morphology of the cells varies with some cells having long processes and others that are epithelioid like.
The cells will aggregate, form clumps and float.
For more information, please contact Cobioer (4008-750-250).

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