MonoMac6（人急性單核細胞白血病細胞）
CBP60521
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| I. General information | |
| Synonyms: | MONO-MAC-6 |
| Background: | established from the peripheral blood of a 64-year-old man with relapsed acute monocytic leukemia (AML FAB M5) in 1985 following myeloid metaplasia; carries t(9;11)(p22;q23) leading to KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion gene; more information on this cell line on the following website: "www.monocytes.de"; sister cell line of MONO-MAC-1 (DSM ACC 252); cells are used for the detection of pyrogens |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | acute monocytic leukemia |
| Gender: | N/A |
| Morphology: | single, round/multiformed cells or small clusters of cells in suspension, sometimes loosely adherent; 1-5% giant cells |
| Growth Mode: | N/A |
| Doubling Time: | ca. 50-60 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS+1%Non Essential Amino Acids (NEAA)+ 1mM sodium pyruvate+10µg/ml insulin MonoMac6完全培養基，# CBP60521M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD4 -, CD13 +, CD14 +, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +; note that CD14 expression depends on cultivation conditions; CD14 positivity cannot be guaranteed; image |
| Fingerprint: | same DNA profile as MONO-MAC-1 using multiplex PCR of minisatellite markers |
| Species: | confirmed as human with IEF of AST, MDH, NP |
| Cytogenetics: | human flat-moded hypotetraploid karyotype with near-diploid (8%) and polyploid (17%) sidelines - 84-90<4n>XX/XXX, -Y, +6, +7, -12, -13, -13, -16, -16, +2mar, t(9;11)(p22;q23)x2, add(10)(p11)x2, add(12)(q21), del(13)(q13q14)der(13)t(13;14)(p11;q12)x2, der(17)t(13;17)(q21;p11)x2 - first cell line shown to carry t(9;11) characteristic of AML M5 |
| Molec. Genetics: | expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
