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EGFR V769_D770insGSV/BaF3

CBP73217

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I. Introduction
Cell Line Name: EGFR V769_D770insGSV/BaF3
Host Cell: Ba/F3
Stability: 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Freeze Medium: 90% FBS+10% DMSO
Complete Culture Medium: RPMI-1640+10%FBS
Mycoplasma Status: Negative
 
II. Background

EGFR V769_D770insGSV results in the insertion of three amino acids in the protein kinase domain of the Egfr protein between amino acids 769 and 770 (UniProt.org). V769_D770insGSV has not been biochemically characterized, but is predicted to result in a gain of function based on the effect of other Egfr exon 20 insertions (PMID: 24353160).

 
III. Representative Data

1. WB of EGFR V769_D770insGSV/BaF3

2. Anti-proliferation assay

 

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