毛片免费毛片一级jjj毛片,日本在线亚州精品视频在线,国产精品国偷自产在线,久久精品色,日韩精品免费电影,欧美综合国产精品日韩一,国产六区

首頁 /藥靶模型 /免疫治療 /Co-Stimulatory /CD27/NFκB-Luc/Jurkat

CD27/NFκB-Luc/Jurkat

CBP74007

詢 價
索取Protocol
產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫
I. Background

CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the T cell co-stimulatory receptors OX40, 4-1BB and herpesvirus entry mediator (HVEM). CD27 is expressed on various types of T cells, B cells and a subset of natural killer cells. It activates NF-κB and MAPK/JNK signaling upon interaction with its TNF-like ligand, CD70, which is expressed by numerous tumor cells. Adaptor proteins TRAF2 and TRAF5 can also stimulate CD27 signaling. Activation of CD27 leads to lymphoid proliferation, differentiation, apoptosis, and the induction of long-term memory. The CD27/CD70 pathway is a key target for the development of treatments for cancer and inflammatory diseases.

 
II. Description
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NF-kB response elements with constitutive expression of human CD27 (also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, Genbank Accession #BC012160)
 
III. Introduction
Host Cell: Jurkat
Expressed gene:

CD27-NFκB-Luciferase

Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, T14, cd27 cell line, cd-27, nfkb, nf-kb
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Peripheral blood
Disease: Childhood T acute lymphoblastic leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
Ⅴ. Representative Data

Figure 1. Recombinant Jurkat stably expressing human CD27.

Figure 2.  Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). Jurkat-CD27-NFκB-Luciferase Reporter cells were stimulated by CD70/CHO cells, the S/B was 26.5-fold.

 

客服

微信

掃一掃,添加二維碼

電話

留言

藥靶模型聯(lián)系方式: 華東銷售經(jīng)理(上海):18240630236 華東銷售經(jīng)理(上海、江蘇、安徽):15715191010 華中&華西銷售經(jīng)理:18071545918 華中&西南銷售經(jīng)理:13871580511 華北銷售經(jīng)理:18628311252 全國銷售經(jīng)理:13816461235
診斷標(biāo)準(zhǔn)品聯(lián)系方式: 華東銷售經(jīng)理:15000320447 華北銷售經(jīng)理:18628311252 華中&華西銷售經(jīng)理:18071545918 華中&西南銷售經(jīng)理:13871580511 全國銷售經(jīng)理:13816461235

掃二維碼

立即提交
剑阁县| 安图县| 弥勒县| 德保县| 美姑县| 安康市| 荔浦县| 镇平县| 廊坊市| 寿阳县| 黑河市| 西贡区| 安陆市| 梅河口市| 沾益县| 民和| 海门市| 礼泉县| 玉田县| 河间市| 微博| 高雄县| 德昌县| 宜都市| 化德县| 青神县| 龙海市| 马山县| 吐鲁番市| 宜宾市| 昔阳县| 建阳市| 鸡西市| 蓬安县| 垫江县| 五华县| 沧州市| 宾川县| 兴山县| 杭州市| 仁寿县|